Karuna A.

Week 3/4:
RE Digest result gel:
6/26/12 VDS

Lane 1- empty
2- 1 kb ladder
3-uncut plasmid
4- sample 1: EcoRI+Ne2buffer
5-sample 2: PvuII+ne2buffer
6- sample 3: ecoRI+PvuII+ne2buffer
7- sample 4- EcoRI+neb buffer
The sample came out properly, with the weights accurately shown above.

Pgbr22 Retry-3 & 4(successful!)

Pgbr22 PCR try 4:


Lane 1: 100 bp ladder
Lane 2-5: PCR KJA samples+dye
UV PCR pgbr22- sources of error: problems in making gel (agarose maybe not completely dissolved), issues in agarose itself, incorrect dilution

Pgbr22 Unsuccessful Try 3:
Lane 1: 1 Kb ladder
Lane 2-5: PCR samples KJA

Week 2:
pmcherry plasmid growth
My First (and Second) PCR-pgbr22 purple protein
Midiprep of pmcherry

Nanodrop Trial 1 Midiprep Final Elution ND1000

Nanodrop Trial 2 Midiprep Final Elution ND1000

DNA sequence still unavailable.

Agarose Gel PCR Trial 1, Unsuccessful. VDS 6/12/2013 PCRpgbr22 KJA MJ Lane 1- 100 bp ladder Lane 2-5: PCR KJA samples Lane 6- 100 bp ladder Lane 7: Sample A Lane 8: Sample B Lane 9: Sample C

100 bp DNA ladder, New England BioLabs

Week 1
Dilution of primer: M13 Reverse (-27) 100 to 20 µM
4 plates of LB Agar Plates made with Amp added
Transformation Efficiency: pmcherry- 120ng/microL
Colonies grown overnight, checked morning after, stored in incubator, then fridge.
PCR with pGBR22; M13 Forward and ReversePrimers; Stored in freezer

6/6: 140 colonies/ng- pmcherry plasmid on LB agar with amp (25 ng)

6/6: 675 colonies/ng- pmcherry plasmid on LB agar with amp (5 ng)

6/6: 3150 colonies/ng- pmcherry plasmid on LB agar with amp (1 ng)